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Band Edge Detection & background subtraction

Band edge detection can be automatic or under full interactive control. Several automatic methods are available that process all lanes with a single mouse click. The Manual Baseline facility permits adjustment of the baseline subtraction for each lane

Band edge detection (red; band centres - blue crosses)

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Part of an analysis window for the above gel  (the minor bands 4,5,14 are not shown on the gel). The dotted lines indicate the band edges found by the software and the purple line is the fitted lane background.

Note: Phoretix software adheres to the gold rule of image analysis & processing: the raw data is unaffected